Blood draw info-Amphibians/reptiles
Amphibians and Reptiles
Blood Collection and Injections
Note: Amphibian blood contains nucleated erythrocytes, leukocytes and thrombocytes. Amphibian erythrocytes are biconvex, oval, and the largest of all vertebrate erythrocytes. Accurate RBC counts are obtained by the use of a hemocytometer and standard counting procedures. The use of automated WBC counters is contraindicated.
Frogs and Toads:
Femoral vein, ventral abdominal vein, or lingual vein, toe web clip as well as terminal cardiac puncture (under anesthesia)
Needle size for most: 25-27 gauge x ¾ inch
IV injections are usually given in the dorsal lymph sac where drugs are rapidly absorbed into the blood
IM injections are usually given in the thigh
- The ventral tail vein, as well as terminal cardiac (under anesthesia)
- Blood is usually collected from the ventral coccygeal vein
Blood is collected from a number of sites including: jugular vein, dorsal coccygeal venous sinus, scapular vein, and brachial vein and artery
- Blood may be collected from the caudal vein (ventrally). The snake is elevated vertically to ensure blood flow to the tail. The needle is gently inserted at a 45 degree angle between the ventral scutes of the tail at a location between 1/2 and 2/3 the distance between the anus and the tip of the tail.
- Inserting the needle close to the ventral midline increases the chance of hitting the centrally located caudal vein or artery.
- The needle should be inserted until a slight resistance from the caudal vertebrae is felt, then back the needle out about 2-5mm, and gently draw back on the plunger
Last Updated on Wednesday, 30 July 2008 07:59